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Leptin receptor Polyclonal Antibody, APC Conjugated

Applications

  • WB
  • IF

Reactivity

  • Rat
Overview
Catalog # bs-0410R-APC
Product Name Leptin receptor Polyclonal Antibody, APC Conjugated
Applications
WB IF
Reactivity Rat
Specifications
Conjugation APC
Host Rabbit
Source KLH conjugated synthetic peptide derived from sheep Leptin R
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Synonyms obl; CD 295; CD295; CD295 antigen; Db; Fa; HuB219; LEP R; LEPR; LEPROT; Leptin receptor fatty; Leptin receptor gene related protein; Leptin receptor precursor; OB R; OB R gene related protein; OB receptor; OB-RGRP; Obr.
Background Leptin receptor (OB-R) is a type I cytokine receptor family protein with significant amino acid sequence identity with gp130, G-CSF receptor, and LIF receptor. An OB-R transcript encodes a soluble form of the receptor. The transcript was reported to be expressed predominantly in regions of the hypothalamus previously thought to be important in body weight regulation. OB-R binds leptin with high affinity and is a potent leptin antagonist. Human OB-R encodes a 1 165 amino acid residue precursor protein with a 22 amino acid residue signal peptide, an 816 amino acid residue extracellular domain, a 21 amino acid residue transmembrane domain and a 303 amino acid residue cytoplasmic domain.
Application Dilution
WB IFIHC-P1:50-200
IF

Frequently asked questions

Which applications is Leptin receptor Polyclonal Antibody, APC Conjugated (bs-0410R-APC) validated for, and at what dilution?

bs-0410R-APC is validated for IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Leptin receptor Polyclonal Antibody, APC Conjugated (bs-0410R-APC) react with?

Tested reactivity: Rat.

How should bs-0410R-APC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Leptin receptor Polyclonal Antibody, APC Conjugated (bs-0410R-APC)?

KLH conjugated synthetic peptide derived from sheep Leptin R

What are the excitation and emission wavelengths of the APC conjugate (bs-0410R-APC)?

APC on bs-0410R-APC: excitation / emission 650nm/660nm.