TIMP-4 Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-0418R-BF647 |
| Product Name | TIMP-4 Polyclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Cow, Horse, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human TIMP-4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7079 |
| Swiss Prot | Q99727 |
| Subcellular location | Secreted |
| Synonyms | Metalloproteinase inhibitor 4; Tissue inhibitor of metalloproteinases 4; TIMP-4; TIMP4 |
| Background | Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7 and MMP-9. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is TIMP-4 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-0418R-BF647) validated for, and at what dilution?
bs-0418R-BF647 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does TIMP-4 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-0418R-BF647) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Cow, Horse, Rabbit.
How should bs-0418R-BF647 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise TIMP-4 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-0418R-BF647)?
KLH conjugated synthetic peptide derived from human TIMP-4
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-0418R-BF647)?
AbBy Fluor-647 on bs-0418R-BF647: excitation / emission 650nm/665nm.