Rabbit Anti-Chicken IgY Antibody, Allophycocyanin Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-0432R-APC |
| Product Name | Rabbit Anti-Chicken IgY Antibody, Allophycocyanin Conjugated |
| Applications |
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Chicken |
| Specifications | |
| Conjugation | Allophycocyanin |
| Host | Rabbit |
| Source | Chicken IgY |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles |
| Target | |
| Synonyms | Egg yolk immunoglobulin; IgY. |
| Background | In chickens, immunoglobulin Y is the functional equivalent to Immunoglobulin G (IgG). Like IgG, it is composed of two light and two heavy chains. Structurally, these two types of immunoglobulin differ primarily in the heavy chains, which in IgY have a molecular mass of about 65,100 atomic mass units (amu), and are thus larger than in IgG. The light chains in IgY, with a molar mass of about 18,700 amu, are somewhat smaller than the light chains in IgG. The molar mass of IgY thus amounts to about 167,000 amu. The steric flexibility of the IgY molecule is less than that of IgG.Functionally, IgY is partially comparable to Immunoglobulin E (IgE), as well as to IgG. However, in contrast to IgG, IgY does not bind to Protein A, to Protein G, or to cellular Fc receptors. Furthermore, IgY does not activate the complement system. The name Immunoglobulin Y was suggested in 1969 by G.A. Leslie and L.W. Clem, after they were able to show differences between the immunoglobulins found in chicken eggs, and immunoglobulin G. Other synonymous names are Chicken IgG, Egg Yolk IgG, and 7S-IgG. |
| Application Dilution | |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Rabbit Anti-Chicken IgY Antibody, Allophycocyanin Conjugated (bs-0432R-APC) validated for, and at what dilution?
bs-0432R-APC is validated for FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Rabbit Anti-Chicken IgY Antibody, Allophycocyanin Conjugated (bs-0432R-APC) react with?
Tested reactivity: Chicken.
How should bs-0432R-APC be stored?
Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
What immunogen was used to raise Rabbit Anti-Chicken IgY Antibody, Allophycocyanin Conjugated (bs-0432R-APC)?
Chicken IgY
What are the excitation and emission wavelengths of the Allophycocyanin conjugate (bs-0432R-APC)?
Allophycocyanin on bs-0432R-APC: excitation / emission 650nm/660nm.