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ACTH (18-39) Polyclonal Antibody, RBITC Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Rat

Predicted Reactivity

  • Human
Overview
Catalog # bs-0442R-RBITC
Product Name ACTH (18-39) Polyclonal Antibody, RBITC Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Rat
Predicted Reactivity Human
Specifications
Conjugation RBITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human ACTH
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 5443
Swiss Prot P01189
Subcellular location Extracellular matrix
Synonyms LPH; MSH; NPP; POC; ACTH; CLIP; Pro-opiomelanocortin; POMC; Corticotropin-lipotropin
Background ACTH stimulates the adrenal glands to release cortisol. MSH (melanocyte-stimulating hormone) increases the pigmentation of skin by increasing melanin production in melanocytes. Beta-endorphin and Met-enkephalin are endogenous opiates.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is ACTH (18-39) Polyclonal Antibody, RBITC Conjugated (bs-0442R-RBITC) validated for, and at what dilution?

bs-0442R-RBITC is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does ACTH (18-39) Polyclonal Antibody, RBITC Conjugated (bs-0442R-RBITC) react with?

Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human.

How should bs-0442R-RBITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise ACTH (18-39) Polyclonal Antibody, RBITC Conjugated (bs-0442R-RBITC)?

KLH conjugated synthetic peptide derived from human ACTH

What are the excitation and emission wavelengths of the RBITC conjugate (bs-0442R-RBITC)?

RBITC on bs-0442R-RBITC: excitation / emission 540nm/625nm.