Help

TSHR Polyclonal Antibody, AbBy Fluor-750 Conjugated

Applications

  • WB
  • IF

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Sheep
  • Pig
  • Horse
  • Chicken
Overview
Catalog # bs-0460r-bf750
Product Name TSHR Polyclonal Antibody, AbBy Fluor-750 Conjugated
Applications
WB IF
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Sheep, Pig, Horse, Chicken
Specifications
Conjugation AbBy Fluor-750
Host Rabbit
Source KLH conjugated synthetic peptide derived from human TSHR
Immunogen Range 501-600/764
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 7253
Swiss Prot P16473
Subcellular location Cell membrane
Synonyms LGR3; CHNG1; hTSHR-I; Thyrotropin receptor; Thyroid-stimulating hormone receptor; TSH-R; TSHR
Background Receptor for thyrothropin. Plays a central role in controlling thyroid cell metabolism. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Also acts as a receptor for thyrostimulin (GPA2+GPB5).
Application Dilution
WB =1:500-2000
IF IF=1:100-500

Frequently asked questions

Which applications is TSHR Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-0460R-BF750) validated for, and at what dilution?

bs-0460R-BF750 is validated for WB (WB=1:500-2000); IF (IF=1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does TSHR Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-0460R-BF750) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Sheep, Pig, Horse, Chicken.

How should bs-0460R-BF750 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise TSHR Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-0460R-BF750)?

KLH conjugated synthetic peptide derived from human TSHR Immunogen range: 501-600/764.

What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bs-0460R-BF750)?

AbBy Fluor-750 on bs-0460R-BF750: excitation / emission 749nm/775nm.