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TSHR Polyclonal Antibody, RBITC Conjugated

Applications

  • WB
  • IF

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Sheep
  • Pig
  • Horse
  • Chicken
Overview
Catalog # bs-0460r-rbitc
Product Name TSHR Polyclonal Antibody, RBITC Conjugated
Applications
WB IF
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Sheep, Pig, Horse, Chicken
Specifications
Conjugation RBITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human TSHR
Immunogen Range 501-600/764
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 7253
Swiss Prot P16473
Subcellular location Cell membrane
Synonyms LGR3; CHNG1; hTSHR-I; Thyrotropin receptor; Thyroid-stimulating hormone receptor; TSH-R; TSHR
Background Receptor for thyrothropin. Plays a central role in controlling thyroid cell metabolism. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Also acts as a receptor for thyrostimulin (GPA2+GPB5).
Application Dilution
WB =1:500-2000
IF IF=1:100-500

Frequently asked questions

Which applications is TSHR Polyclonal Antibody, RBITC Conjugated (bs-0460R-RBITC) validated for, and at what dilution?

bs-0460R-RBITC is validated for WB (WB=1:500-2000); IF (IF=1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does TSHR Polyclonal Antibody, RBITC Conjugated (bs-0460R-RBITC) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Sheep, Pig, Horse, Chicken.

How should bs-0460R-RBITC be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise TSHR Polyclonal Antibody, RBITC Conjugated (bs-0460R-RBITC)?

KLH conjugated synthetic peptide derived from human TSHR Immunogen range: 501-600/764.

What are the excitation and emission wavelengths of the RBITC conjugate (bs-0460R-RBITC)?

RBITC on bs-0460R-RBITC: excitation / emission 570nm/595nm.