human IgA Polyclonal Antibody, PerCP Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-0474R-PerCP |
| Product Name | human IgA Polyclonal Antibody, PerCP Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | PerCP |
| Host | Rabbit |
| Source | purified human Serum IgA |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Secreted |
| Synonyms | NULL |
| Background | Human IgA (immunoglobulin A) is a glycosylated protein of 160 kDa and is produced as a monomer or as a J chain linked dimer. Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces such as saliva, gastrointestinal secretion, bronchial fluids and milk. Mucosal IgA plays a major role in host defence by neutralising infectious agents at mucosal surfaces. The production is usually local and antigen specific IgA producing B cells can be found in regions under the lamina propria where they mature into dimeric IgA producing plasma cells. IgA deficiency is the most common immunodeficiency that may affect both serum and mucosal produced IgA. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is human IgA Polyclonal Antibody, PerCP Conjugated (bs-0474R-PerCP) validated for, and at what dilution?
bs-0474R-PerCP is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does human IgA Polyclonal Antibody, PerCP Conjugated (bs-0474R-PerCP) react with?
Tested reactivity: Human.
How should bs-0474R-PerCP be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise human IgA Polyclonal Antibody, PerCP Conjugated (bs-0474R-PerCP)?
purified human Serum IgA
What are the excitation and emission wavelengths of the PerCP conjugate (bs-0474R-PerCP)?
PerCP on bs-0474R-PerCP: excitation / emission 482nm/677nm.