BrdU(Proliferation Marker) Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-0489R-BF647 |
| Product Name | BrdU(Proliferation Marker) Polyclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Species independent |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | BrdU conjugated to KLH |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Nucleus |
| Synonyms | Bromodeoxyuridine; Bromodeoxyuridine; 5-Bromo-2-deoxyuridine; 5-BrdU; proliferation Marker. |
| Background | The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal is an accurate and comprehensive method to quantitate the degree of DNA-synthesis.BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is BrdU(Proliferation Marker) Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-0489R-BF647) validated for, and at what dilution?
bs-0489R-BF647 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does BrdU(Proliferation Marker) Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-0489R-BF647) react with?
Tested reactivity: Species independent.
How should bs-0489R-BF647 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise BrdU(Proliferation Marker) Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-0489R-BF647)?
BrdU conjugated to KLH
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-0489R-BF647)?
AbBy Fluor-647 on bs-0489R-BF647: excitation / emission 650nm/665nm.