BrdU Polyclonal Antibody, Biotin Conjugated

Applications

  • IHC-P
  • IHC-F

Reactivity

  • Human
  • Rat
  • Others
Overview
Catalog # bs-0489R-Biotin
Product Name BrdU Polyclonal Antibody, Biotin Conjugated
Applications IHC-P, IHC-F
Reactivity Human, Rat, Others
Specifications
Conjugation Biotin
Host Rabbit
Source BrdU conjugated to KLH
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer HEPES with 0.15M NaCl, 0.01% BSA, 0.03% sodium azide, and 50% glycerol.
Storage Condition Store at 4°C for up to 2 weeks. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Target
Subcellular location Nucleus
Synonyms Bromodeoxyuridine; Bromodeoxyuridine; 5-Bromo-2-deoxyuridine; 5-BrdU; proliferation Marker.
Background The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal is an accurate and comprehensive method to quantitate the degree of DNA-synthesis.BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis
Application Dilution
IHC-P 1:200-400
IHC-F 1:100-500