ETFA Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-0494R-RBITC |
| Product Name | ETFA Polyclonal Antibody, RBITC Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Xenopus laevis |
| Predicted Reactivity | Human, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ETFA |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2108 |
| Subcellular location | Cytoplasm |
| Synonyms | ETF-alpha;Electron transfer flavoprotein subunit alpha;electron-transfer-flavoprotein, alpha polypeptide;mitochondrial;Alpha ETF;Alpha-ETF;Electron transfer flavoprotein alpha polypeptide;Electron transfer flavoprotein alpha subunit;Electron transfer flavoprotein subunit alpha;Electron transfer flavoprotein subunit alpha mitochondrial;Electron transfer flavoprotein subunit alpha, mitochondrial;Electron transferring flavoprotein alpha polypeptide;EMA;ETFA;ETFA_HUMAN;GA2. |
| Background | ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is ETFA Polyclonal Antibody, RBITC Conjugated (bs-0494R-RBITC) validated for, and at what dilution?
bs-0494R-RBITC is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ETFA Polyclonal Antibody, RBITC Conjugated (bs-0494R-RBITC) react with?
Tested reactivity: Mouse, Xenopus laevis. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Sheep, Pig, Horse, Rabbit.
How should bs-0494R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ETFA Polyclonal Antibody, RBITC Conjugated (bs-0494R-RBITC)?
KLH conjugated synthetic peptide derived from human ETFA
What are the excitation and emission wavelengths of the RBITC conjugate (bs-0494R-RBITC)?
RBITC on bs-0494R-RBITC: excitation / emission 540nm/625nm.