Dnmt3a Polyclonal Antibody

Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Anti-Dnmt3a Polyclonal Antibody, Unconjugated (bs-0497R) at 1:200, followed by conjugation to the secondary antibody and DAB staining

Image provided by One World Lab validation program. SH-SY5Y cells probed with Dnmt3a Polyclonal Antibody (bs-0497R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.

U-87MG cells(black) were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Dnmt3a Antibody(bs-R)at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).