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Sodium hydrogen exchanger 1 Polyclonal Antibody, PE Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Cow
  • Pig
  • Horse
  • Chicken
  • Rabbit
Overview
Catalog # bs-0505R-PE
Product Name Sodium hydrogen exchanger 1 Polyclonal Antibody, PE Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Cow, Pig, Horse, Chicken, Rabbit
Specifications
Conjugation PE
Host Rabbit
Source KLH conjugated synthetic peptide derived from human NHE1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 6548
Swiss Prot P19634
Subcellular location Cell membrane
Synonyms APNH; NHE1; LIKNS; NHE-1; PPP1R143; Sodium/hydrogen exchanger 1; Na(+)/H(+) antiporter, amiloride-sensitive; Na(+)/H(+) exchanger 1; Solute carrier family 9 member 1; SLC9A1; APNH1
Background Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction.
Application Dilution
WB 1:300-5000
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Frequently asked questions

Which applications is Sodium hydrogen exchanger 1 Polyclonal Antibody, PE Conjugated (bs-0505R-PE) validated for, and at what dilution?

bs-0505R-PE is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Sodium hydrogen exchanger 1 Polyclonal Antibody, PE Conjugated (bs-0505R-PE) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Pig, Horse, Chicken, Rabbit.

How should bs-0505R-PE be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Sodium hydrogen exchanger 1 Polyclonal Antibody, PE Conjugated (bs-0505R-PE)?

KLH conjugated synthetic peptide derived from human NHE1

What are the excitation and emission wavelengths of the PE conjugate (bs-0505R-PE)?

PE on bs-0505R-PE: excitation / emission 496,564nm/578nm.