ATF3 Polyclonal Antibody, AbBy Fluor-405 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-0519R-BF405 |
| Product Name | ATF3 Polyclonal Antibody, AbBy Fluor-405 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-405 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ATF3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 467 |
| Swiss Prot | P18847 |
| Subcellular location | Nucleus |
| Synonyms | Cyclic AMP-dependent transcription factor ATF-3; cAMP-dependent transcription factor ATF-3; Activating transcription factor 3; ATF3 |
| Background | This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | FCM1:20-100 |
Frequently asked questions
Which applications is ATF3 Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-0519R-BF405) validated for, and at what dilution?
bs-0519R-BF405 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ATF3 Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-0519R-BF405) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-0519R-BF405 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ATF3 Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-0519R-BF405)?
KLH conjugated synthetic peptide derived from human ATF3
What are the excitation and emission wavelengths of the AbBy Fluor-405 conjugate (bs-0519R-BF405)?
AbBy Fluor-405 on bs-0519R-BF405: excitation / emission 401nm/421nm.