PKA alpha + beta Polyclonal Antibody, PerCP Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-0520R-PerCP-100uL |
| Product Name | PKA alpha + beta Polyclonal Antibody, PerCP Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | PerCP |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from bovine PKA |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 282323 |
| Swiss Prot | P05131 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | cAMP-dependent protein kinase catalytic subunit beta; PKA C-beta; PRKACB |
| Background | Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, and differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis (By similarity). |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is PKA alpha + beta Polyclonal Antibody, PerCP Conjugated (bs-0520R-PerCP) validated for, and at what dilution?
bs-0520R-PerCP is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PKA alpha + beta Polyclonal Antibody, PerCP Conjugated (bs-0520R-PerCP) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-0520R-PerCP be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PKA alpha + beta Polyclonal Antibody, PerCP Conjugated (bs-0520R-PerCP)?
KLH conjugated synthetic peptide derived from bovine PKA
What are the excitation and emission wavelengths of the PerCP conjugate (bs-0520R-PerCP)?
PerCP on bs-0520R-PerCP: excitation / emission 482nm/677nm.