CD45 Polyclonal Antibody, AbBy Fluor-680 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-0522R-BF680 |
| Product Name | CD45 Polyclonal Antibody, AbBy Fluor-680 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(ICC)
|
| Specificity | The antibody is predicted to work with Mouse and Rat samples, but with decreased sensitivity compared to human samples. |
| Reactivity | Human |
| Specifications | |
| Conjugation | AbBy Fluor-680 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CD45 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5788 |
| Swiss Prot | P08575 |
| Subcellular location | Cell membrane |
| Synonyms | LCA; LY5; B220; CD45; L-CA; T2; CD45R; GP18; Receptor-type tyrosine-protein phosphatase C; Leukocyte common antigen; PTPRC |
| Background | Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity). |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is CD45 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-0522R-BF680) validated for, and at what dilution?
bs-0522R-BF680 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CD45 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-0522R-BF680) react with?
Tested reactivity: Human.
How should bs-0522R-BF680 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CD45 Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-0522R-BF680)?
KLH conjugated synthetic peptide derived from human CD45
What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bs-0522R-BF680)?
AbBy Fluor-680 on bs-0522R-BF680: excitation / emission 679nm/702nm.