IL18 Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-0529R-Cy5-100uL |
| Product Name | IL18 Polyclonal Antibody, Cy5 Conjugated |
| Applications |
IF(IHC-P)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from rat IL-18 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 29197 |
| Synonyms | interferon gamma-inducing factor IG; Interferon gamma-inducing factor; N-gamma-inducing factor; Interleukin-1 gamma; Iboctadekin; N gamma inducing factor; IG; IL 1 gamma; IL 18; IL 1g; IL1 gamma; IL18 protein; IL1F4; IL1g; IL1gamma; IL4; Interferon gamma inducing factor; Interleukin 1 gamma; Interleukin 18; Interleukin18; MGC12320; IL18_MOUSE. |
| Background | IL18 (IFN-gamma-inducing factor, IGIF) is a novel pro-inflammatory cytokine that augments natural killer (NK) activity in spleen cells.This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity.IL18 also has other biological properties such as stimulation of Fas ligand expression, and induction of CC and CXC chemokines. IL18 increases production of competent HIV. IL18 is related to the IL1 family. One of the IL18 receptor chains is the IL1 receptor-related protein, a member of the IL 1R family. Like IL1 , IL18 is first synthesized as a leaderless precursor requiring the IL1 converting enzyme (ICE) for cleavage into an active molecule. IL18 may play a role in tissue injury in inflammatory reactions. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is IL18 Polyclonal Antibody, Cy5 Conjugated (bs-0529R-Cy5) validated for, and at what dilution?
bs-0529R-Cy5 is validated for IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does IL18 Polyclonal Antibody, Cy5 Conjugated (bs-0529R-Cy5) react with?
Tested reactivity: Human.
How should bs-0529R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise IL18 Polyclonal Antibody, Cy5 Conjugated (bs-0529R-Cy5)?
KLH conjugated synthetic peptide derived from rat IL-18
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-0529R-Cy5)?
Cy5 on bs-0529R-Cy5: excitation / emission 625,650nm/670nm.