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CDKN1C Polyclonal Antibody, AbBy Fluor-405 Conjugated

Applications

  • FCM
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Cow
  • Sheep
Overview
Catalog # bs-0538R-BF405
Product Name CDKN1C Polyclonal Antibody, AbBy Fluor-405 Conjugated
Applications
FCM IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Predicted Reactivity Cow, Sheep
Specifications
Conjugation AbBy Fluor-405
Host Rabbit
Source KLH conjugated synthetic peptide derived from rat Cdkn1c
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 246060
Subcellular location Nucleus
Synonyms p57; Kip2; p57KIP2
Background This gene is imprinted, with preferential expression of the maternal allele. The encoded protein is a tight-binding, strong inhibitor of several G1 cyclin/Cdk complexes and a negative regulator of cell proliferation. Mutations in this gene are implicated in sporadic cancers and Beckwith-Wiedemann syndorome, suggesting that this gene is a tumor suppressor candidate. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2010].
Application Dilution
FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is CDKN1C Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-0538R-BF405) validated for, and at what dilution?

bs-0538R-BF405 is validated for FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CDKN1C Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-0538R-BF405) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Cow, Sheep.

How should bs-0538R-BF405 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CDKN1C Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-0538R-BF405)?

KLH conjugated synthetic peptide derived from rat Cdkn1c

What are the excitation and emission wavelengths of the AbBy Fluor-405 conjugate (bs-0538R-BF405)?

AbBy Fluor-405 on bs-0538R-BF405: excitation / emission 401nm/421nm.