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Endostatin Polyclonal Antibody, PerCP Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
Overview
Catalog # bs-0547R-PerCP-100uL
Product Name Endostatin Polyclonal Antibody, PerCP Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse
Predicted Reactivity Rat
Specifications
Conjugation PerCP
Host Rabbit
Source KLH conjugated synthetic peptide derived from human COL18A1/Endostatin
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 80781
Swiss Prot P39060
Subcellular location Secreted, Extracellular matrix
Synonyms KS; KNO; KNO1; Collagen alpha-1(XVIII) chain; COL18A1
Background COLA18A probably plays a major role in determining the retinal structure as well as in the closure of the neural tube. Endostatin potently inhibits endothelial cell proliferation and angiogenesis. May inhibit angiogenesis by binding to the heparan sulfate proteoglycans involved in growth factor signaling.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Endostatin Polyclonal Antibody, PerCP Conjugated (bs-0547R-PerCP) validated for, and at what dilution?

bs-0547R-PerCP is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Endostatin Polyclonal Antibody, PerCP Conjugated (bs-0547R-PerCP) react with?

Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat.

How should bs-0547R-PerCP be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Endostatin Polyclonal Antibody, PerCP Conjugated (bs-0547R-PerCP)?

KLH conjugated synthetic peptide derived from human COL18A1/Endostatin

What are the excitation and emission wavelengths of the PerCP conjugate (bs-0547R-PerCP)?

PerCP on bs-0547R-PerCP: excitation / emission 482nm/677nm.