Collagen 3 Polyclonal Antibody

Lane 1: Human MCF-7 cell lysates; Lane 2: Human Hela cell lysates probed with Collagen 3 Polyclonal Antibody, Unconjugated (bs-0549R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Lane 1: Human HeLa cell lysates; Lane 2: Human MCF-7 cell lysates probed with Collagen III Polyclonal Antibody, Unconjugated (bs-0549R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Human HeLa cell lysates; Lane 2: Human MCF-7 cell lysates probed with Collagen III Polyclonal Antibody, Unconjugated (bs-0549R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

Reactivity

Human
Mouse
Rat
Dog
Rabbit

Predicted Reactivity

Cow
Chicken

Overview
Catalog #	bs-0549R
Product Name	Collagen 3 Polyclonal Antibody
Applications	WB
Specificity	This antibody is produced with an immunogen contained within the COLFI propeptide region, it will only recognize the full length Collagen III protein and not the mature form.
Reactivity	Human, Mouse, Rat, Dog, Rabbit
Predicted Reactivity	Cow, Chicken
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Collagen alpha 1(III) chain (C-terminal propeptide)
Immunogen Range	1301-1400/1466
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	1281
Swiss Prot	P02461
Subcellular location	Secreted, Extracellular matrix
Synonyms	EDS4A; Collagen alpha-1(III) chain; COL3A1
Background	Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of GPR56 in the developing brain and binding to GPR56 inhibits neuronal migration and activates the RhoA pathway by coupling GPR56 to GNA13 and possibly GNA12.
Application Dilution
WB	1:300-5000

Applications

Reactivity

Predicted Reactivity

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