Pro-Collagen 1 Polyclonal Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-0578r-100uL |
| Product Name | Pro-Collagen 1 Polyclonal Antibody |
| Applications |
WB
ELISA
FCM
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | This antibody is produced using a peptide that is found in the C-terminal propeptide region. |
| Reactivity | Human, Mouse, Rat, Rabbit |
| Predicted Reactivity | Dog, Cow, Sheep, Chicken |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Collagen I C-terminal propeptide |
| Immunogen Range | 1321-1400/1464 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 1277 |
| Subcellular location | Secreted, Extracellular matrix |
| Synonyms | Collagen alpha-1(I) chain; Alpha-1 type I collagen; COL1A1, Pro-collagen 1. |
| Background | This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. Type I collagen levels have been linked to bone growth and formation. Levels of the C-terminal propeptide are indicative of collagen production in vivo. Elevated levels of C-terminal propeptide have been shown in diseases associated with high levels of bone turnover, including Pagets disease, hyperthyroidism, primary hyperparathyroidism and renal osteodystrophy. Slightly elevated levels of C-terminal propeptide have also been documented in some women in early menopause. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| FCM | 1:20-100 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Pro-Collagen 1 Polyclonal Antibody (bs-0578R) validated for, and at what dilution?
bs-0578R is validated for WB (1:300-5000); ELISA (1:500-1000); FCM (1:20-100); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Pro-Collagen 1 Polyclonal Antibody (bs-0578R) react with?
Tested reactivity: Human, Mouse, Rat, Rabbit. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Chicken.
How should bs-0578R be stored?
Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Pro-Collagen 1 Polyclonal Antibody (bs-0578R)?
KLH conjugated synthetic peptide derived from human Collagen I C-terminal propeptide Immunogen range: 1321-1400/1464.