DPPA2 Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-0597R-BF488 |
| Product Name | DPPA2 Polyclonal Antibody, AbBy Fluor-488 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
|
| Predicted Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from mouse DPPA2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 151871 |
| Subcellular location | Nucleus |
| Synonyms | Developmental Pluripotency Associated 2; developmental pluripotency associated gene2; PESCRG1; Pluripotent embryonic stem cell related protein 1; cancer/testis antigen 100; CT100; developmental pluripotency-associated gene 2; developmental pluripotency-associated protein 2; ECAT15-2; embryonic stem cell ESC associated transcript 15-2; PESCRG1; Pluripotent embryonic stem cell related protein 1; Pluripotent embryonic stem cell-related gene 1; Pluripotent embryonic stem cell-related gene 1 protein . |
| Background | SCRG1 (scrapie-responsive protein 1) is a 98 amino acid secreted protein that belongs to the SCRG1 family. The SCRG1 protein contains a 20-amino acid signal peptide, and is expressed abundantly in the central nervous system of human adult, but not at all in fetal brain. The protein is targeted to the Golgi apparatus and large dense-core vesicles/secretory granules in neurons. High levels of SCRG1 transcripts are also observed in testis and aorta. SCRG1 is associated with neurodegenerative changes observed in transmissible spongiform encephalopathies. It may play a role in host response to prion-associated infections. The SCRG1 protein may be partly included in the membrane or secreted by the cells due to its hydrophobic N-terminus. The human and mouse SCRG1 proteins share 83% sequence identity. The SCRG1 gene is conserved in chimpanzee, cow, mouse, rat and chicken, and maps to human chromosome 4q34.1. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
Frequently asked questions
Which applications is DPPA2 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-0597R-BF488) validated for, and at what dilution?
bs-0597R-BF488 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-0597R-BF488 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise DPPA2 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-0597R-BF488)?
KLH conjugated synthetic peptide derived from mouse DPPA2
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-0597R-BF488)?
AbBy Fluor-488 on bs-0597R-BF488: excitation / emission 499nm/519nm.