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Adiponectin receptor 2 Antibody, Cy7 Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Pig
  • Chicken
Overview
Catalog # bs-0611R-Cy7
Product Name Adiponectin receptor 2 Antibody, Cy7 Conjugated
Applications
WB FCM IF(IHC-P) IF(IHC-F) IF(ICC)
Specificity This antibody may cross react with Adiponectin receptor 1 due to sequence similarity.
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Pig, Chicken
Specifications
Conjugation Cy7
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Adiponectin receptor 2
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 79602
Swiss Prot Q86V24
Subcellular location Cell membrane
Synonyms PAQR2; ACDCR2; Adiponectin receptor protein 2; Progestin and adipoQ receptor family member II; ADIPOR2
Background Receptor for globular and full-length adiponectin (APM1), an essential hormone secreted by adipocytes that acts as an antidiabetic. Probably involved in metabolic pathways that regulate lipid metabolism such as fatty acid oxidation. Mediates increased AMPK, PPARA ligand activity, fatty acid oxidation and glucose uptake by adiponectin. Has some intermediate-affinity receptor activity for both globular and full-length adiponectin.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Adiponectin receptor 2 Antibody, Cy7 Conjugated (bs-0611R-Cy7) validated for, and at what dilution?

bs-0611R-Cy7 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Adiponectin receptor 2 Antibody, Cy7 Conjugated (bs-0611R-Cy7) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Pig, Chicken.

How should bs-0611R-Cy7 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Adiponectin receptor 2 Antibody, Cy7 Conjugated (bs-0611R-Cy7)?

KLH conjugated synthetic peptide derived from human Adiponectin receptor 2

What are the excitation and emission wavelengths of the Cy7 conjugate (bs-0611R-Cy7)?

Cy7 on bs-0611R-Cy7: excitation / emission 743nm/767nm.