Estrogen Sulfotransferase Polyclonal Antibody

$Formalin-fixed and paraffin embedded human placenta labeled with Rabbit Anti SULT1E1\/Estrogen Sulfotranferase Polyclonal Antibody, Unconjugated (bs-0629R) at 1:200 followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded human placenta labeled with Rabbit Anti SULT1E1\/Estrogen Sulfotranferase Polyclonal Antibody, Unconjugated (bs-0629R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti SULT1E1\/Estrogen Sulfotranferase Polyclonal Antibody, Unconjugated (bs-0629R) at 1:200 followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti SULT1E1\/Estrogen Sulfotranferase Polyclonal Antibody, Unconjugated (bs-0629R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

$HepG2 probed with Estrogen Sulfotransferase Polyclonal Antibody, Unconjugated (bs-0629R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.\r\n$

HepG2 probed with Estrogen Sulfotransferase Polyclonal Antibody, Unconjugated (bs-0629R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.\r\n

$MCF-7 Cell lysates probed with Estrogen Sulfotransferase Polyclonal Antibody, Unconjugated (bs-0629R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.\r\n$

MCF-7 Cell lysates probed with Estrogen Sulfotransferase Polyclonal Antibody, Unconjugated (bs-0629R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.\r\n

Applications

WB
ELISA
IHC-P

Reactivity

Human

Predicted Reactivity

Mouse
Rat
Dog
Pig
Horse
Rabbit

Overview
Catalog #	bs-0629R
Product Name	Estrogen Sulfotransferase Polyclonal Antibody
Applications	WB ELISA IHC-P
Reactivity	Human
Predicted Reactivity	Mouse, Rat, Dog, Pig, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human Estrogen Sulfotranferase
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	6783
Swiss Prot	P49888
Subcellular location	Cytoplasm
Synonyms	EST; STE; EST-1; ST1E1; Estrogen sulfotransferase; Sulfotransferase 1E1; Sulfotransferase, estrogen-preferring; SULT1E1
Background	Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of estradiol and estrone. May play a role in the regulation of estrogen receptor activity by metabolizing free estradiol. Maximally sulfates beta-estradiol and estrone at concentrations of 2 nM. Also sulfates dehydroepiandrosterone, pregnenolone, ethinylestradiol, equalenin, diethylstilbesterol and 1-naphthol, at significantly higher concentrations; however, cortisol, testosterone and dopamine are not sulfated.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400