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Integrin alpha 4 Polyclonal Antibody

Formalin-fixed and paraffin embedded mouse lung labeled with Rabbit Anti Integrin alpha 4\/CD49d\/Integrin \u03b14 Polyclonal Antibody, Unconjugated (bs-0641R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded mouse lung labeled with Rabbit Anti Integrin alpha 4\/CD49d\/Integrin \u03b14 Polyclonal Antibody, Unconjugated (bs-0641R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Human U937 cells probed with Integrin alpha 4\/CD49d Antibody, Unconjugated (bs-0641R) at 0.2ug for 30 minutes followed by incubation with a PE Conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Human U937 cells probed with Integrin alpha 4\/CD49d Antibody, Unconjugated (bs-0641R) at 0.2ug for 30 minutes followed by incubation with a PE Conjugated secondary (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
U-937 (Positive) and MCF7 (Negative control) cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Integrin alpha 4\/CD49dAntibody( bs-0641R) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).\r\n
U-937 (Positive) and MCF7 (Negative control) cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Integrin alpha 4\/CD49dAntibody( bs-0641R) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).\r\n
THP-1 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Integrin alpha 4 Polyclonal Antibody(bs-0641R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
THP-1 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Integrin alpha 4 Polyclonal Antibody(bs-0641R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Lane 1: Normal human Jurkat cell lysates; Lane 2: Jurkat treated with PMA 125ng\/ml 30min probed with Integrin alpha 4 Polyclonal Antibody, Unconjugated (bs-0641R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Lane 1: Normal human Jurkat cell lysates; Lane 2: Jurkat treated with PMA 125ng\/ml 30min probed with Integrin alpha 4 Polyclonal Antibody, Unconjugated (bs-0641R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Lane 1: Human A549 cell lysates; Lane 2: Human MCF-7 cell lysates probed with Integrin alpha 4 Polyclonal Antibody, Unconjugated (bs-0641R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Lane 1: Human A549 cell lysates; Lane 2: Human MCF-7 cell lysates probed with Integrin alpha 4 Polyclonal Antibody, Unconjugated (bs-0641R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37\u02daC.
Bioss unconjugated antibody vial

Applications

  • WB
  • ELISA
  • IHC-P
  • FCM

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Cow
  • Horse
  • Rabbit
Overview
Catalog # bs-0641R-100uL
Product Name Integrin alpha 4 Polyclonal Antibody
Applications
WB ELISA IHC-P FCM
Reactivity Human
Predicted Reactivity Mouse, Rat, Cow, Horse, Rabbit
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Integrin alpha 4
Immunogen Range 551-650/1032
Clonality Polyclonal
Isotype IgG
Concentration Lot Dependent
Purification purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 3676
Swiss Prot P13612
Subcellular location Cell membrane
Synonyms IA4; CD49D; Integrin alpha-4; CD49 antigen-like family member D; Integrin alpha-IV; VLA-4 subunit alpha; ITGA4
Background Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
FCM 1:20-100

Frequently asked questions

Which applications is Integrin alpha 4 Polyclonal Antibody (bs-0641R) validated for, and at what dilution?

bs-0641R is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Integrin alpha 4 Polyclonal Antibody (bs-0641R) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Cow, Horse, Rabbit.

How should bs-0641R be stored?

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Integrin alpha 4 Polyclonal Antibody (bs-0641R)?

KLH conjugated synthetic peptide derived from human Integrin alpha 4 Immunogen range: 551-650/1032.

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