CD4 Polyclonal Antibody, AbBy Fluor-350 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-0647R-BF350 |
| Product Name | CD4 Polyclonal Antibody, AbBy Fluor-350 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat, Pig, Chicken |
| Predicted Reactivity | Dog, Cow, Sheep, Guinea Pig |
| Specifications | |
| Conjugation | AbBy Fluor-350 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from Human CD4 (cytoplasmic domain) |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 24932 |
| Swiss Prot | P01730 |
| Subcellular location | Cell membrane |
| Synonyms | T-cell surface glycoprotein CD4; Cd4; T-cell surface antigen T4/Leu-3; W3/25 antigen |
| Background | Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is CD4 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-0647R-BF350) validated for, and at what dilution?
bs-0647R-BF350 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CD4 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-0647R-BF350) react with?
Tested reactivity: Human, Mouse, Rat, Pig, Chicken. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Guinea Pig.
How should bs-0647R-BF350 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CD4 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-0647R-BF350)?
KLH conjugated synthetic peptide derived from Human CD4 (cytoplasmic domain)
What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bs-0647R-BF350)?
AbBy Fluor-350 on bs-0647R-BF350: excitation / emission 346nm/442nm.