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Connexin 43 Polyclonal Antibody, PerCP Conjugated

Applications

  • WB
  • FCM

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Cow
  • Chicken
Overview
Catalog # bs-0651R-PerCP-100uL
Product Name Connexin 43 Polyclonal Antibody, PerCP Conjugated
Applications
WB FCM
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Cow, Chicken
Specifications
Conjugation PerCP
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Connexin-43
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 2697
Swiss Prot P17302
Subcellular location Cytoplasm, Cell membrane, Extracellular matrix
Synonyms HSS; CMDR; CX43; GJAL; ODDD; AVSD3; HLHS1; Gap junction alpha-1 protein; Connexin-43; Gap junction 43 kDa heart protein; GJA1
Background Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract (By similarity).
Application Dilution
WB 1:50-200
FCM 1:500-1000

Frequently asked questions

Which applications is Connexin 43 Polyclonal Antibody, PerCP Conjugated (bs-0651R-PerCP) validated for, and at what dilution?

bs-0651R-PerCP is validated for WB (1:50-200); FCM (1:500-1000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Connexin 43 Polyclonal Antibody, PerCP Conjugated (bs-0651R-PerCP) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Chicken.

How should bs-0651R-PerCP be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Connexin 43 Polyclonal Antibody, PerCP Conjugated (bs-0651R-PerCP)?

KLH conjugated synthetic peptide derived from human Connexin-43

What are the excitation and emission wavelengths of the PerCP conjugate (bs-0651R-PerCP)?

PerCP on bs-0651R-PerCP: excitation / emission 482nm/677nm.