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Growth hormone receptor Polyclonal Antibody, PE Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Cow
  • Sheep
  • Pig
  • Chicken
Overview
Catalog # bs-0654R-PE
Product Name Growth hormone receptor Polyclonal Antibody, PE Conjugated
Applications
WB FCM IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Cow, Sheep, Pig, Chicken
Specifications
Conjugation PE
Host Rabbit
Source KLH conjugated synthetic peptide derived from human GHR
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 2690
Swiss Prot P10912
Subcellular location Cell membrane
Synonyms GHBP; Growth hormone receptor; GH receptor; Somatotropin receptor; GHR
Background Receptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway (By similarity). The soluble form (GHBP) acts as a reservoir of growth hormone in plasma and may be a modulator/inhibitor of GH signaling. Isoform 2 up-regulates the production of GHBP and acts as a negative inhibitor of GH signaling.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Frequently asked questions

Which applications is Growth hormone receptor Polyclonal Antibody, PE Conjugated (bs-0654R-PE) validated for, and at what dilution?

bs-0654R-PE is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Growth hormone receptor Polyclonal Antibody, PE Conjugated (bs-0654R-PE) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Pig, Chicken.

How should bs-0654R-PE be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Growth hormone receptor Polyclonal Antibody, PE Conjugated (bs-0654R-PE)?

KLH conjugated synthetic peptide derived from human GHR

What are the excitation and emission wavelengths of the PE conjugate (bs-0654R-PE)?

PE on bs-0654R-PE: excitation / emission 496,564nm/578nm.