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HMGB1 Polyclonal Antibody, APC Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Cow
Overview
Catalog # bs-0664R-APC
Product Name HMGB1 Polyclonal Antibody, APC Conjugated
Applications
WB FCM IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Predicted Reactivity Cow
Specifications
Conjugation APC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human HMGB1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 3146
Swiss Prot P09429
Subcellular location Nucleus
Synonyms HMG1; HMG3; SBP-1; High mobility group protein B1; High mobility group protein 1; HMG-1; HMGB1
Background DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells (By similarity).
Application Dilution
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Frequently asked questions

Which applications is HMGB1 Polyclonal Antibody, APC Conjugated (bs-0664R-APC) validated for, and at what dilution?

bs-0664R-APC is validated for WB, FCM, IF(IHC-P), IF(IHC-F), IF(ICC).

Which species does HMGB1 Polyclonal Antibody, APC Conjugated (bs-0664R-APC) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Cow.

How should bs-0664R-APC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise HMGB1 Polyclonal Antibody, APC Conjugated (bs-0664R-APC)?

KLH conjugated synthetic peptide derived from human HMGB1