Fibronectin/FN1 Polyclonal Antibody, PE-Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-0666R-PE-Cy5 |
| Product Name | Fibronectin/FN1 Polyclonal Antibody, PE-Cy5 Conjugated |
| Applications |
WB
|
| Reactivity | Human, Mouse, Rat, Cow |
| Predicted Reactivity | Dog |
| Specifications | |
| Conjugation | PE-Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from mouse Fibronectin |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 14268 |
| Swiss Prot | P11276 |
| Subcellular location | Secreted, Extracellular matrix |
| Synonyms | Fn; Fn-1; E3327I9; Fibronectin; Fn1 |
| Background | Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts. Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling (By similarity). |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Fibronectin/FN1 Polyclonal Antibody, PE-Cy5 Conjugated (bs-0666R-PE-Cy5) validated for, and at what dilution?
bs-0666R-PE-Cy5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Fibronectin/FN1 Polyclonal Antibody, PE-Cy5 Conjugated (bs-0666R-PE-Cy5) react with?
Tested reactivity: Human, Mouse, Rat, Cow. Predicted reactivity (expected from sequence homology but not yet tested): Dog.
How should bs-0666R-PE-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Fibronectin/FN1 Polyclonal Antibody, PE-Cy5 Conjugated (bs-0666R-PE-Cy5)?
KLH conjugated synthetic peptide derived from mouse Fibronectin
What are the excitation and emission wavelengths of the PE-Cy5 conjugate (bs-0666R-PE-Cy5)?
PE-Cy5 on bs-0666R-PE-Cy5: excitation / emission 488nm/670nm.