Dnmt1 Polyclonal Antibody, Biotin Conjugated

Applications

  • WB
  • ELISA
  • IHC-P

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
  • Chicken
Overview
Catalog # bs-0678R-Biotin
Product Name Dnmt1 Polyclonal Antibody, Biotin Conjugated
Applications WB, ELISA, IHC-P
Specificity This antibody will not recongize isoform 3.
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Cow, Sheep, Pig, Horse, Chicken
Specifications
Conjugation Biotin
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Dnmt1
Immunogen Range 21-120/1616
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 1786
Swiss Prot P26358
Subcellular location Nucleus
Synonyms AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN; DNA (cytosine-5)-methyltransferase 1; Dnmt1; CXXC-type zinc finger protein 9; DNA methyltransferase HsaI; DNA MTase HsaI; M.HsaI
Background Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9.
Application Dilution
WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400