PDK4 Polyclonal Antibody, APC-Cy7 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-0682R-APC-Cy7 |
| Product Name | PDK4 Polyclonal Antibody, APC-Cy7 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | APC-Cy7 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Pdk4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5166 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | FLJ40832; Pyruvate dehydrogenase [lipoamide] kinase isozyme 4 mitochondrial; Pyruvate dehydrogenase kinase 4; Pyruvate dehydrogenase kinase isoform 4; Pyruvate dehydrogenase kinase isozyme 4 mitochondrial. |
| Background | Pdk4 (pyruvate dehydrogenase kinase, isozyme 4) mediates glucose oxidation through inhibitory phosphorylation of the pyruvate dehydrogenase complex. The pyruvate dehydrogenase complex (PDC) is a mitochondrial matrix enzyme that plays an key role in aerobic energy metabolism by catalyzing the oxidative decarboxylation of pyruvate to form acetyl-CoA for subsequent breakdown in the Krebs cycle. The enzymatic activity of the mammalian PDC is regulated through phosphorylation and desphosphorylation of the E1?subunit of the complex. The enzymes responsible for the phosphorylation and inactivation of the PDC are pyruvate dehydrogenase kinases (PDK1-4). [TISSUE SPECI FICITY] Ubiquitous; highest levels of expression in heart and skeletal muscle. Belongs to the PDK/BCKDK protein kinase family. Contains 1 histidine kinase domain. |
| Application Dilution | |
| WB | |
| IF(IHC-P) | |
| IF(IHC-F) | |
| IF(ICC) | |
Frequently asked questions
Which applications is PDK4 Polyclonal Antibody, APC-Cy7 Conjugated (bs-0682R-APC-Cy7) validated for, and at what dilution?
bs-0682R-APC-Cy7 is validated for WB, IF(IHC-P), IF(IHC-F), IF(ICC).
Which species does PDK4 Polyclonal Antibody, APC-Cy7 Conjugated (bs-0682R-APC-Cy7) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-0682R-APC-Cy7 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PDK4 Polyclonal Antibody, APC-Cy7 Conjugated (bs-0682R-APC-Cy7)?
KLH conjugated synthetic peptide derived from human Pdk4