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CD147 Polyclonal Antibody, PE Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Cow
  • Pig
  • Chicken
  • Rabbit
  • GuineaPig
Overview
Catalog # bs-0684R-PE-100uL
Product Name CD147 Polyclonal Antibody, PE Conjugated
Applications
WB FCM IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Cow, Pig, Chicken, Rabbit, GuineaPig
Specifications
Conjugation PE
Host Rabbit
Source KLH conjugated synthetic peptide derived from human CD147/TCSF
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 682
Swiss Prot P35613
Subcellular location Cell membrane
Synonyms M6; OK; 5F7; TCSF; CD147; EMMPRIN; Basigin; Collagenase stimulatory factor; Extracellular matrix metalloproteinase inducer; Leukocyte activation antigen M6; OK blood group antigen; Tumor cell-derived collagenase stimulatory factor; BSG; UNQ655/PRO21383
Background Plays an important role in targeting the monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to the plasma membrane. Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Frequently asked questions

Which applications is CD147 Polyclonal Antibody, PE Conjugated (bs-0684R-PE) validated for, and at what dilution?

bs-0684R-PE is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CD147 Polyclonal Antibody, PE Conjugated (bs-0684R-PE) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Pig, Chicken, Rabbit, GuineaPig.

How should bs-0684R-PE be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CD147 Polyclonal Antibody, PE Conjugated (bs-0684R-PE)?

KLH conjugated synthetic peptide derived from human CD147/TCSF

What are the excitation and emission wavelengths of the PE conjugate (bs-0684R-PE)?

PE on bs-0684R-PE: excitation / emission 496,564nm/578nm.

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