PTEN Polyclonal Antibody, PE-Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-0686R-PE-Cy5-100uL |
| Product Name | PTEN Polyclonal Antibody, PE-Cy5 Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Mouse, Rat |
| Specifications | |
| Conjugation | PE-Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PTEN |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5728 |
| Swiss Prot | P60484 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | Bannayan Zonana; BZS a; MGC11227; MHAM; MMAC1; Multiple hamartoma (Cowden syndrome); Mutated in Multiple Advanced Cancers 1; Phosphatase and Tensin Homolog; Phosphatidylinositol 345 trisphosphate 3 phosphatase and dual specificity protein phosphatase PTEN; Phosphatidylinositol 345 trisphosphate 3 phosphatase; Platelet antigen BR; PTEN1; Tensin homolog; TEP1; VLA 2 Receptor Alpha Subunit; 10q23del; Bannayan Zonana syndrome; BZS; DEC; GLM2; MMAC1 phosphatase and tensin homolog deleted on chromosome 10; Phosphatase and Tensin Homolog; Phosphatase and Tensin Homolog; Phosphatase and tensin like protein; Phosphatidylinositol 345 trisphosphate 3 phosphatase and dual specificity protein phosphatase PTEN; Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN; PTEN_HUMAN. |
| Background | This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. [provided by RefSeq, Jul 2008] |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is PTEN Polyclonal Antibody, PE-Cy5 Conjugated (bs-0686R-PE-Cy5) validated for, and at what dilution?
bs-0686R-PE-Cy5 is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PTEN Polyclonal Antibody, PE-Cy5 Conjugated (bs-0686R-PE-Cy5) react with?
Tested reactivity: Mouse, Rat.
How should bs-0686R-PE-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PTEN Polyclonal Antibody, PE-Cy5 Conjugated (bs-0686R-PE-Cy5)?
KLH conjugated synthetic peptide derived from human PTEN
What are the excitation and emission wavelengths of the PE-Cy5 conjugate (bs-0686R-PE-Cy5)?
PE-Cy5 on bs-0686R-PE-Cy5: excitation / emission 488nm/670nm.