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OAS1 Polyclonal Antibody

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-OAS1 Polyclonal Antibody, Unconjugated (bs-0687R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human lung carcinoma labeled with Anti-OAS1 Polyclonal Antibody, Unconjugated (bs-0687R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Hela cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with OAS1 Antibody(bs-0687R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).
Hela cells were\u00a0fixed with 4% PFA for 10min at room temperature,permeabilized with\u00a090% ice-cold methanol for 20 min at -20\u2103,\u00a0and incubated in 5% BSA blocking buffer\u00a0for 30 min at room temperature. Cells were then stained with OAS1 Antibody(bs-0687R)at 1:100 dilution\u00a0in blocking buffer and\u00a0incubated for 30 min at\u00a0room temperature,\u00a0washed twice with 2%BSA in PBS,\u00a0followed by\u00a0secondary antibody incubation\u00a0for 40 min\u00a0at\u00a0room temperature. Acquisitions of 20,000 events were performed.\u00a0Cells stained with primary antibody\u00a0(green), and isotype control (orange).
Bioss unconjugated antibody vial

Applications

  • WB
  • IHC-P
  • IHC-F
  • IF
  • Flow-Cyt

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Pig
Overview
Catalog # bs-0687R-100uL
Product Name OAS1 Polyclonal Antibody
Applications
WB IHC-P IHC-F IF FLOW-CYT
Reactivity Human
Predicted Reactivity Mouse, Pig
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human OAS1
Clonality Polyclonal
Isotype IgG
Concentration 1mg/ml
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 4938
Swiss Prot P00973
Subcellular location Cytoplasm, Nucleus
Synonyms 2',5'-oligoadenylate synthetase 1; (2 5')oligo(A) synthetase 1 ; (2 5')oligo(A) synthetase 1; 2 5 Oligoadenylate Synthetase 1; 2 5A synthetase 1; 2' 5' oligo A synthetase 1; 2' 5' oligoadenylate synthetase 1; 2' 5' oligoisoadenylate synthetase 1; 2'5' oligo A synthetase 1; 2'5' oligoadenylate synthetase 1; 2'5' oligoisoadenylate synthetase 1; E18/E16; IFI 4; IFI4; OAS 1; OIAS; OIASI; p46/p42 OAS; OAS1_HUMAN.
Application Dilution
WB ELISAELISA=1:5000-10000
IHC-P FCMFCM=1:100
IHC-F
IF
Flow-Cyt

Frequently asked questions

Which applications is OAS1 Polyclonal Antibody (bs-0687R) validated for, and at what dilution?

bs-0687R is validated for ELISA (ELISA=1:5000-10000); FCM (FCM=1:100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does OAS1 Polyclonal Antibody (bs-0687R) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Pig.

How should bs-0687R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise OAS1 Polyclonal Antibody (bs-0687R)?

KLH conjugated synthetic peptide derived from human OAS1

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