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LDLR Polyclonal Antibody, PE Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)

Reactivity

  • Human
  • Mouse

Predicted Reactivity

  • Rat
  • Dog
  • Cow
  • Pig
  • Horse
  • Rabbit
  • GuineaPig
Overview
Catalog # bs-0705R-PE
Product Name LDLR Polyclonal Antibody, PE Conjugated
Applications
WB FCM IF(IHC-P)
Reactivity Human, Mouse
Predicted Reactivity Rat, Dog, Cow, Pig, Horse, Rabbit, GuineaPig
Specifications
Conjugation PE
Host Rabbit
Source KLH conjugated synthetic peptide derived from human LDL-R
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 3949
Swiss Prot P01130
Subcellular location Cytoplasm, Cell membrane
Synonyms FH; FHC; LDLCQ2; Low-density lipoprotein receptor; LDL receptor; LDLR
Background Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. In case of HIV-1 infection, functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IF(IHC-P)

Frequently asked questions

Which applications is LDLR Polyclonal Antibody, PE Conjugated (bs-0705R-PE) validated for, and at what dilution?

bs-0705R-PE is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does LDLR Polyclonal Antibody, PE Conjugated (bs-0705R-PE) react with?

Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Pig, Horse, Rabbit, GuineaPig.

How should bs-0705R-PE be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise LDLR Polyclonal Antibody, PE Conjugated (bs-0705R-PE)?

KLH conjugated synthetic peptide derived from human LDL-R

What are the excitation and emission wavelengths of the PE conjugate (bs-0705R-PE)?

PE on bs-0705R-PE: excitation / emission 496,564nm/578nm.