NF-M Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-0710R-BF488 |
| Product Name | NF-M Polyclonal Antibody, AbBy Fluor-488 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Cow, Pig |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from rat NF-M |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 24588 |
| Swiss Prot | P12839 |
| Subcellular location | Cytoplasm |
| Synonyms | Nfm; Nef3; Neurofilament medium polypeptide; NF-M; 16 kDa neurofilament protein; Neurofilament 3; Neurofilament triplet M protein; Nefm |
| Background | Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is NF-M Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-0710R-BF488) validated for, and at what dilution?
bs-0710R-BF488 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does NF-M Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-0710R-BF488) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Cow, Pig.
How should bs-0710R-BF488 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NF-M Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-0710R-BF488)?
KLH conjugated synthetic peptide derived from rat NF-M
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-0710R-BF488)?
AbBy Fluor-488 on bs-0710R-BF488: excitation / emission 499nm/519nm.