Estrogen Receptor alpha Antibody, Biotin Conjugated

Applications

  • WB
  • IHC-P
  • IHC-F

Reactivity

  • Human
  • Rat

Predicted Reactivity

  • Mouse
Overview
Catalog # bs-0725R-Biotin
Product Name Estrogen Receptor alpha Antibody, Biotin Conjugated
Applications WB, IHC-P, IHC-F
Reactivity Human, Rat
Predicted Reactivity Mouse
Specifications
Conjugation Biotin
Host Rabbit
Source KLH conjugated synthetic peptide derived from rat ER-alpha
Immunogen Range 501-600/600
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 24890
Swiss Prot P06211
Subcellular location Cytoplasm, Nucleus, Cell membrane
Synonyms Esr; ER-alpha; RNESTROR; Estrogen receptor; ER; Estradiol receptor; Nuclear receptor subfamily 3 group A member 1; Esr1; Estr; Nr3a1
Background Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA-binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements; the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3 (By similarity).
Application Dilution
WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500