PEDF Polyclonal Antibody, AbBy Fluor-680 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-0731R-BF680 |
| Product Name | PEDF Polyclonal Antibody, AbBy Fluor-680 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Dog, Cow, Pig |
| Specifications | |
| Conjugation | AbBy Fluor-680 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from mouse PEDF |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 20317 |
| Swiss Prot | P97298 |
| Subcellular location | Secreted |
| Synonyms | Pedf; Sdf3; EPC-1; Pedfl; AI195227; Pigment epithelium-derived factor; Caspin; Serpin F1; Stromal cell-derived factor 3; SDF-3; Serpinf1 |
| Background | Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is PEDF Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-0731R-BF680) validated for, and at what dilution?
bs-0731R-BF680 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PEDF Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-0731R-BF680) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Dog, Cow, Pig.
How should bs-0731R-BF680 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PEDF Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-0731R-BF680)?
KLH conjugated synthetic peptide derived from mouse PEDF
What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bs-0731R-BF680)?
AbBy Fluor-680 on bs-0731R-BF680: excitation / emission 679nm/702nm.