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ADAMTS7(NT) Polyclonal Antibody, Cy5.5 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-0733R-Cy5.5-100uL
Product Name ADAMTS7(NT) Polyclonal Antibody, Cy5.5 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Specifications
Conjugation Cy5.5
Host Rabbit
Source KLH conjugated synthetic peptide derived from mouse ADAMTS7
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 108153
Swiss Prot Q68SA9
Subcellular location Secreted, Cell membrane, Extracellular matrix
Synonyms ADAM-TS7; ADAMTS7B; A disintegrin and metalloproteinase with thrombospondin motifs 7; ADAM-TS 7; ADAMTS-7; COMPase; Adamts7
Background Metalloprotease that may play a role in the degradation of COMP. Binds 1 zinc ion per subunit. pH dependence: Optimum pH is between 7.5 and 9.5. [Subcellular location] Secreted, extracellular space, extracellular matrix (By similarity). Note=Also found associated with the external cell surface[Tissue specificity] Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Detected in meniscus, bone, tendon, cartilage, synovium, fat and ligaments. Up-regulated in articular cartilage and synovium from arthritis patients.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is ADAMTS7(NT) Polyclonal Antibody, Cy5.5 Conjugated (BS-0733R-CY5-5) validated for, and at what dilution?

BS-0733R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does ADAMTS7(NT) Polyclonal Antibody, Cy5.5 Conjugated (BS-0733R-CY5-5) react with?

Tested reactivity: Human, Mouse, Rat.

How should BS-0733R-CY5-5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise ADAMTS7(NT) Polyclonal Antibody, Cy5.5 Conjugated (BS-0733R-CY5-5)?

KLH conjugated synthetic peptide derived from mouse ADAMTS7

What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-0733R-CY5-5)?

Cy5.5 on BS-0733R-CY5-5: excitation / emission 675nm/694nm.