CDKN1A/p21 Polyclonal Antibody, Biotin Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-0741r-biotin |
| Product Name | CDKN1A/p21 Polyclonal Antibody, Biotin Conjugated |
| Applications |
ELISA
IHC-P
IHC-F
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Chicken |
| Specifications | |
| Conjugation | Biotin |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CDKN1A |
| Immunogen Range | 101-164/164 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Gene ID | 12575 |
| Swiss Prot | P38936 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | P21; CDKI; CIP1; SDI1; Waf1; mda6; CAP2; Cdkn1; p21WAF; p21Cip1; Cyclin-dependent kinase inhibitor 1; CDK-interacting protein 1; Melanoma differentiation-associated protein; Cdkn1a |
| Background | May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex (By similarity). |
| Application Dilution | |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is CDKN1A/p21 Polyclonal Antibody, Biotin Conjugated (bs-0741R-Biotin) validated for, and at what dilution?
bs-0741R-Biotin is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CDKN1A/p21 Polyclonal Antibody, Biotin Conjugated (bs-0741R-Biotin) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Chicken.
How should bs-0741R-Biotin be stored?
Store at -20°C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CDKN1A/p21 Polyclonal Antibody, Biotin Conjugated (bs-0741R-Biotin)?
KLH conjugated synthetic peptide derived from human CDKN1A Immunogen range: 101-164/164.