Vimentin Polyclonal Antibody

Applications

  • WB
  • ELISA
  • FCM
  • IHC-P
  • IHC-F
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)
  • ICC

Reactivity

  • Human
  • Mouse
  • Rat
  • Bovine
  • Pig
  • Others
  • Monkey

Predicted Reactivity

  • Cow
  • Chicken
  • Goat
Overview
Catalog # bs-0756R
Product Name Vimentin Polyclonal Antibody
Applications WB, ELISA, FCM, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC), ICC
Specificity KO-Validated
Reactivity Human, Mouse, Rat, Bovine, Pig, Others, Monkey
Predicted Reactivity Cow, Chicken, Goat
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Vimentin
Immunogen Range 371-466/466
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 7431
Swiss Prot P08670
Subcellular location Cytoplasm, Extracellular matrix
Synonyms HEL113; CTRCT3; Vimentin; VIM
Background Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
Application Dilution
WB 1:500-2000
ELISA 1:5000-10000
FCM 1:100
IHC-P 1:100-500
IHC-F 1:100-500
IF(IHC-P) 1:100-500
IF(IHC-F) 1:100-500
IF(ICC) 1:100
ICC 1:100