MSH2 Polyclonal Antibody, Biotin Conjugated
ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.", "Category"=>"Conjugated Primary Antibody", "Clonality"=>"Polyclonal", "Concentration"=>"1ug/ul", "Conjugation"=>"Biotin", "Cross Reactive Species"=>"Human, Mouse", "Datasheet URL"=>"https://www.biossusa.com/store/datasheets/bs-0758R-Biotin", "Excitation Emission"=>"N/A", "Gene ID"=>"4436", "Gene ID Link"=>"Entrez Gene: 4436\"", "Host"=>"Rabbit", "Isotype"=>"IgG", "Lead Time"=>"10 to 15 business days.", "Modification"=>"Unmodified", "Notes"=>"AbBy Fluor Push", "Predicted Reactivity"=>"Cow", "Product URL"=>"www.biossusa.com/products/bs-0758R-Biotin", "Purification"=>"Purified by Protein A.", "Size"=>"100ul", "Source"=>"KLH conjugated synthetic peptide derived from human MSH-2", "Storage"=>"Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. Store at -20C for 12 months.", "Storage Buffer"=>"Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.", "Storage Condition"=>"Store at -20C for 12 months.", "Subcellular Locations"=>"Nucleus", "Swiss Prot"=>"P43246", "Swiss Prot Link"=>"Swiss Prot: P43246\"", "Synonyms"=>"FCC1; COCA1; HNPCC; LCFS2; HNPCC1; DNA mismatch repair protein Msh2; hMSH2; MutS protein homolog 2; MSH2", "Target Protein"=>"MSH2"} -->
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-0758R-Biotin-100uL |
| Product Name | MSH2 Polyclonal Antibody, Biotin Conjugated |
| Applications |
ELISA
IHC-P
IHC-F
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Cow |
| Specifications | |
| Conjugation | Biotin |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human MSH-2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C for 12 months. |
| Target | |
| Gene ID | 4436 |
| Swiss Prot | P43246 |
| Subcellular location | Nucleus |
| Synonyms | FCC1; COCA1; HNPCC; LCFS2; HNPCC1; DNA mismatch repair protein Msh2; hMSH2; MutS protein homolog 2; MSH2 |
| Background | Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis. |
| Application Dilution | |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
Frequently asked questions
Which applications is MSH2 Polyclonal Antibody, Biotin Conjugated (bs-0758R-Biotin) validated for, and at what dilution?
bs-0758R-Biotin is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does MSH2 Polyclonal Antibody, Biotin Conjugated (bs-0758R-Biotin) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Cow.
How should bs-0758R-Biotin be stored?
Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise MSH2 Polyclonal Antibody, Biotin Conjugated (bs-0758R-Biotin)?
KLH conjugated synthetic peptide derived from human MSH-2