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Lpin1 protein Polyclonal Antibody, AbBy Fluor-488 Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse

Predicted Reactivity

  • Rat
Overview
Catalog # bs-0760R-BF488
Product Name Lpin1 protein Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse
Predicted Reactivity Rat
Specifications
Conjugation AbBy Fluor-488
Host Rabbit
Source KLH conjugated synthetic peptide derived from mouse Lpin 1
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 14245
Swiss Prot Q91ZP3
Subcellular location Cytoplasm, Nucleus
Synonyms fld; Lipin1; Kiaa0188; mKIAA0188; 4631420P06; Phosphatidate phosphatase LPIN1; Fatty liver dystrophy protein; Lipin-1; Lpin1
Background Plays important roles in controlling the metabolism of fatty acids at differents levels. Acts as a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis. Acts also as nuclear transcriptional coactivator for PPARGC1A/PPARA regulatory pathway to modulate lipid metabolism gene expression. Is involved in adipocyte differentiation. Isoform 1 is recruited at the mitochondrion outer membrane and is involved in mitochondrial fission by converting phosphatidic acid to diacylglycerol.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Lpin1 protein Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-0760R-BF488) validated for, and at what dilution?

bs-0760R-BF488 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Lpin1 protein Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-0760R-BF488) react with?

Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat.

How should bs-0760R-BF488 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Lpin1 protein Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-0760R-BF488)?

KLH conjugated synthetic peptide derived from mouse Lpin 1

What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-0760R-BF488)?

AbBy Fluor-488 on bs-0760R-BF488: excitation / emission 499nm/519nm.