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Midnolin Polyclonal Antibody, PerCP Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Mouse
  • Rat
  • Zebrafish

Predicted Reactivity

  • Human
  • Dog
  • Cow
  • Pig
  • Guinea Pig
Overview
Catalog # bs-0761R-PerCP
Product Name Midnolin Polyclonal Antibody, PerCP Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Mouse, Rat, Zebrafish
Predicted Reactivity Human, Dog, Cow, Pig, Guinea Pig
Specifications
Conjugation PerCP
Host Rabbit
Source KLH conjugated synthetic peptide derived from human MIDN
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 90007
Swiss Prot Q504T8
Subcellular location Cytoplasm, Nucleus
Synonyms Midnolin; Midbrain nucleolar protein; MIDN
Background May be involved in regulation of genes related to neurogenesis in the nucleolus.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Midnolin Polyclonal Antibody, PerCP Conjugated (bs-0761R-PerCP) validated for, and at what dilution?

bs-0761R-PerCP is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Midnolin Polyclonal Antibody, PerCP Conjugated (bs-0761R-PerCP) react with?

Tested reactivity: Mouse, Rat, Zebrafish. Predicted reactivity (expected from sequence homology but not yet tested): Human, Dog, Cow, Pig, Guinea Pig.

How should bs-0761R-PerCP be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Midnolin Polyclonal Antibody, PerCP Conjugated (bs-0761R-PerCP)?

KLH conjugated synthetic peptide derived from human MIDN

What are the excitation and emission wavelengths of the PerCP conjugate (bs-0761R-PerCP)?

PerCP on bs-0761R-PerCP: excitation / emission 482nm/677nm.