CD3E Polyclonal Antibody, PE Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-0765R-PE |
| Product Name | CD3E Polyclonal Antibody, PE Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | PE |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from mouse CD3 epsilon |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 12501 |
| Swiss Prot | P22646 |
| Subcellular location | Cell membrane |
| Synonyms | CD3; T3e; AI54783; CD3epsilon; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; CD3e |
| Background | The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | |
Frequently asked questions
Which applications is CD3E Polyclonal Antibody, PE Conjugated (bs-0765R-PE) validated for, and at what dilution?
bs-0765R-PE is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CD3E Polyclonal Antibody, PE Conjugated (bs-0765R-PE) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-0765R-PE be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CD3E Polyclonal Antibody, PE Conjugated (bs-0765R-PE)?
KLH conjugated synthetic peptide derived from mouse CD3 epsilon
What are the excitation and emission wavelengths of the PE conjugate (bs-0765R-PE)?
PE on bs-0765R-PE: excitation / emission 496,564nm/578nm.