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CD4 Polyclonal Antibody, PE Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-0766R-PE
Product Name CD4 Polyclonal Antibody, PE Conjugated
Applications
WB FCM IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Specifications
Conjugation PE
Host Rabbit
Source KLH conjugated synthetic peptide derived from mouse CD4
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 12504
Swiss Prot P06332
Subcellular location Cell membrane
Synonyms L3T4; Ly-4; T-cell surface glycoprotein CD4; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; CD4
Background Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Frequently asked questions

Which applications is CD4 Polyclonal Antibody, PE Conjugated (bs-0766R-PE) validated for, and at what dilution?

bs-0766R-PE is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CD4 Polyclonal Antibody, PE Conjugated (bs-0766R-PE) react with?

Tested reactivity: Human, Mouse, Rat.

How should bs-0766R-PE be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CD4 Polyclonal Antibody, PE Conjugated (bs-0766R-PE)?

KLH conjugated synthetic peptide derived from mouse CD4

What are the excitation and emission wavelengths of the PE conjugate (bs-0766R-PE)?

PE on bs-0766R-PE: excitation / emission 496,564nm/578nm.