CaMK2 alpha Polyclonal Antibody, HRP Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-0771R-HRP-100uL |
| Product Name | CaMK2 alpha Polyclonal Antibody, HRP Conjugated |
| Applications |
WB
IHC-P
IHC-F
|
| Specificity | The peptide immunogen is highly conserved between CaMKII alpha, beta, gamma, and delta. |
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | HRP |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CaMK2 alpha |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 816 |
| Swiss Prot | Q13554 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | CAMK2B; Calcium/calmodulin-dependent protein kinase type II subunit beta; CaM kinase II subunit beta; CaMK-II subunit beta; CAM2; CAMK2; CAMKB; CAMK; CAMK-II; CAMKG; CAMK2G; CAMK2A; CAMK2D; CAMKD |
| Background | Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca2+/calmodulin-binding and autophosphorylation, and is involved in dendritic spine and synapse formation, neuronal plasticity and regulation of sarcoplasmic reticulum Ca2+ transport in skeletal muscle. In neurons, plays an essential structural role in the reorganization of the actin cytoskeleton during plasticity by binding and bundling actin filaments in a kinase-independent manner. This structural function is required for correct targeting of CaMK2A, which acts downstream of NMDAR to promote dendritic spine and synapse formation and maintain synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning. In developing hippocampal neurons, promotes arborization of the dendritic tree and in mature neurons, promotes dendritic remodeling. Participates in the modulation of skeletal muscle function in response to exercise. In slow-twitch muscles, is involved in regulation of sarcoplasmic reticulum (SR) Ca2+ transport and in fast-twitch muscle participates in the control of Ca2+ release from the SR through phosphorylation of triadin, a ryanodine receptor-coupling factor, and phospholamban (PLN/PLB), an endogenous inhibitor of SERCA2A/ATP2A2. |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC-P | ELISA1:500-1000 |
| IHC-F | IHC-P1:200-400 |
Frequently asked questions
Which applications is CaMK2 alpha Polyclonal Antibody, HRP Conjugated (bs-0771R-HRP) validated for, and at what dilution?
bs-0771R-HRP is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CaMK2 alpha Polyclonal Antibody, HRP Conjugated (bs-0771R-HRP) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-0771R-HRP be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CaMK2 alpha Polyclonal Antibody, HRP Conjugated (bs-0771R-HRP)?
KLH conjugated synthetic peptide derived from human CaMK2 alpha