Collagen IV Polyclonal Antibody, PE Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-0806R-PE |
| Product Name | Collagen IV Polyclonal Antibody, PE Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | This antibody has 79% cross reactivity with Collagen alpha-5(IV) chain. |
| Reactivity | Human |
| Specifications | |
| Conjugation | PE |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from mouse Collagen alpha-1(IV) chain |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 12826 |
| Swiss Prot | P02463 |
| Subcellular location | Secreted, Cell membrane, Extracellular matrix |
| Synonyms | Bru; Raw; Svc; Col4a-1; Del(8)44H; Collagen alpha-1(IV) chain; Col4a1 |
| Background | Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation. Inhibits expression of hypoxia-inducible factor 1alpha and ERK1/2 and p38 MAPK activation. Ligand for alpha1/beta1 integrin (By similarity). |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | |
| IF(IHC-F) | |
| IF(ICC) | |
Frequently asked questions
Which applications is Collagen IV Polyclonal Antibody, PE Conjugated (bs-0806R-PE) validated for, and at what dilution?
bs-0806R-PE is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Collagen IV Polyclonal Antibody, PE Conjugated (bs-0806R-PE) react with?
Tested reactivity: Human.
How should bs-0806R-PE be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Collagen IV Polyclonal Antibody, PE Conjugated (bs-0806R-PE)?
KLH conjugated synthetic peptide derived from mouse Collagen alpha-1(IV) chain
What are the excitation and emission wavelengths of the PE conjugate (bs-0806R-PE)?
PE on bs-0806R-PE: excitation / emission 496,564nm/578nm.