IL-1 Beta Polyclonal Antibody

THP-1 (H) cells were treated with or without TPA (80nM) for 24 h,then treated with (100ng\/ml) lipopolysaccharide (LPS) for 6 h, then with (300ng\/ml) Brefeldin A added after 3 h, 25 \u03bcg total protein per lane of cell lysates (see on figure) probed with IL-1 Beta polyclonal antibody, unconjugated (bs-0812R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Paraformaldehyde-fixed, paraffin embedded Human acute villusitis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with IL-1 Beta Polyclonal Antibody, Unconjugated (bs-0812R) at 1:1000 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

Paraformaldehyde-fixed, paraffin embedded Human purulent appendicitis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with IL-1 Beta Polyclonal Antibody, Unconjugated (bs-0812R) at 1:1000 overnight at 4\u00b0C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.