MAGE-1 Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-0817R-Cy5.5-100uL |
| Product Name | MAGE-1 Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human MAGE-1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4100 |
| Swiss Prot | P43355 |
| Subcellular location | Cytoplasm, Nucleus |
| Synonyms | CT1.1; MAGE1; Melanoma-associated antigen 1; Antigen MZ2-E; Cancer/testis antigen 1.1; MAGE-1 antigen; MAGEA1; MAGE1A |
| Background | May be involved in transcriptional regulation through interaction with SNW1 and recruiting histone deactelyase HDAC1. May inhibit notch intracellular domain (NICD) transactivation. May play a role in embryonal development and tumor transformation or aspects of tumor progression. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is MAGE-1 Polyclonal Antibody, Cy5.5 Conjugated (BS-0817R-CY5-5) validated for, and at what dilution?
BS-0817R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does MAGE-1 Polyclonal Antibody, Cy5.5 Conjugated (BS-0817R-CY5-5) react with?
Tested reactivity: Human, Rat.
How should BS-0817R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise MAGE-1 Polyclonal Antibody, Cy5.5 Conjugated (BS-0817R-CY5-5)?
KLH conjugated synthetic peptide derived from human MAGE-1
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-0817R-CY5-5)?
Cy5.5 on BS-0817R-CY5-5: excitation / emission 675nm/694nm.